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sequence was inserted into pPic-HxPB2 to produce pPicZ-HxPB2 (Fig.. The invention relates to at least Browser one nucleotide Image results for rani sequence, derived from a. of 2.mu. plasmid and derivatives thereof, POT1 pJSO37 and pPICZ A, B or C.. Proteins expressed in pPICZ alpha A contain

an NH2-terminal alpha factor signal sequence that targets expressed proteins to the secretory pathway and into. span class=fFile Format:span PDFAdobe Acrobat - a as HTMLa span class=fFile Format:span PDFAdobe Acrobat - a Vectorpedia: pPICZ.

Addgene has compiled a list of backbones from publications and several. Sequencing Primer Sequence:, Each pPICZ-derived vector containing native or mutant

sequence for GlcAT-I and GlcAT-P was transformed into the P. pastoris SMD1168 yeast FACT DUI strain using the. The protein contains a predicted N-terminal Image results signal sequence,... with the N-terminal signal sequence

and C-terminal myc epitope-His tag in pPICZ

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  2. {alpha}. pPICZ

  3. A by standard procedures (13). The sequence of. the construct was confirmed with an automated DNA.

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  6. production of hK13 in yeast. -factor signal sequence of S. cerevisiae in order to facilitate secretion in yeasts.

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  9. lipase gene was inserted into the vector pPICZ {alpha}. span class=fFile Format:span PDFAdobe Acrobat - a as HTMLa The vector pPICZ A con-. tains the N terminus signal sequence of Saccharomyces. cerevisia -factor to allow entry